Fig 1: Tanycyte protrusions are in close proximity to different neural cells. (a–l) High-magnification z-stack images (×63) showing the distribution of tdTomato (red, a–l), and dextran fluorescence (green, a,b), HuC/HuD immunoreactivity (green, c,d), CD31 and NeuN immunoreactivity (cyan and green respectively, e), GFAP immunoreactivity (green, f–g), TMEM119 immunoreactivity (green, h,i), NG2 immunoreactivity (green, j), OLIG2 immunoreactivity (green, k), and myelin basic protein (MBP) immunoreactivity (green, l) with DAPI counterstaining (blue, a–d and f–l) in coronal sections in the arcuate nucleus, in Zone 2. Pictures (a–l) are the maximal intensity projections of z-stack acquisition. Inset on the top in a–l panels shows the orthogonal view on the horizontal line; inset on the right in a–l panels shows the orthogonal view on the vertical line. Arrows and arrowheads indicate the site of contact in the coronal sections and in the orthogonal views, respectively. Low magnifications images are available upon request. Scale bars = 10 µm in a–l [Color figure can be viewed at wileyonlinelibrary.com]